Isolation and characterization of 20 microsatellite markers from Carcharhinus leucas (bull shark) and cross-amplification in Galeocerdo cuvier (tiger shark), Carcharhinus obscurus (dusky shark) and Carcharhinus plumbeus (sandbar shark)

Agathe Pirog (1) , Antonin Blaison (1, 2) , Sébastien Jaquemet (3, 1) , Marc Soria (2) , Hélène Magalon (3, 1)

1 ENTROPIE [Réunion] - Ecologie marine tropicale dans les Océans Pacifique et Indien
2 UMR EME - Ecosystèmes Marins Exploités
3 LabEX CORAIL - Laboratoire d'Excellence CORAIL

With the development of genetics methods, it becomes possible to study the population structure and some aspects of the reproductive behaviour of endangered sharks. Here we describe the isolation of 20 polymorphic microsatellite markers in the bull shark Carcharhinus leucas (Carcharhinidae) and their characteristics. Two to 10 alleles per locus were detected. Observed and expected heterozygosities ranged from 0.00 to 0.78 and from 0.05 to 0.80, respectively. Four markers showed deviations from Hardy–Weinberg equilibrium; among them, three showed presence of null alleles. No linkage disequilibrium was detected among any of the loci. Moreover, four, 11 and 19 of these 20 markers successfully cross-amplified in the tiger shark Galeocerdo cuvier, the sandbar shark Carcharhinus plumbeus and the dusky shark Carcharhinus obscurus, respectively.

Domaines: Biodiversité et Ecologie

Agathe Pirog, Antonin Blaison, Sébastien Jaquemet, Marc Soria, Hélène Magalon. Isolation and characterization of 20 microsatellite markers from Carcharhinus leucas (bull shark) and cross-amplification in Galeocerdo cuvier (tiger shark), Carcharhinus obscurus (dusky shark) and Carcharhinus plumbeus (sandbar shark). Conservation Genetics Resources, 2015, 7 (1), ⟨10.1007/s12686-014-0308-3⟩. ⟨hal-01253773⟩