Isolation and characterisation of 26 microsatellite loci from a widespread tropical hydrozoan, Macrorhynchia phoenicea (Leptothecata, Aglaopheniidae), and cross-amplification in closely related species

Bautisse Postaire (1, 2) , D. Aurelle (3) , Chloé A.-F. Annie-France Bourmaud (1, 2) , J. Henrich Bruggemann (1, 2) , Hélène Magalon (2, 1)

1 ENTROPIE [Réunion] - Ecologie marine tropicale dans les Océans Pacifique et Indien
2 LabEX CORAIL - Laboratoire d'Excellence CORAIL
3 IMBE - Institut méditerranéen de biodiversité et d'écologie marine et continentale

We isolated and characterized 26 microsatellite loci for Macrorhynchia phoenicea (Busk, 1852), a rather common tropical hydrozoan from the Indo-Pacific. The number of alleles per locus ranged from 4 to 24. The observed heterozygosity ranged from 0.000 to 0.970 and the expected heterozygosity from 0.029 to 0.833. Ten loci were at HardyeWeinberg equilibrium. No pair of loci presented linkage disequilibrium. Transferability of up to 18 loci was positive across four other Aglaopheniidae species from different genera. These loci will be used in studying reef population connectivity for these particular species at the scale of the Indo-Pacific, a promising but little explored research field.

Domaines: Biodiversité et Ecologie

Bautisse Postaire, D. Aurelle, Chloé A.-F. Annie-France Bourmaud, J. Henrich Bruggemann, Hélène Magalon. Isolation and characterisation of 26 microsatellite loci from a widespread tropical hydrozoan, Macrorhynchia phoenicea (Leptothecata, Aglaopheniidae), and cross-amplification in closely related species. Biochemical Systematics and Ecology, 2015, 62, ⟨10.1016/j.bse.2015.08.011⟩. ⟨hal-01253779⟩