Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa)

Bautisse Postaire (1, 2, 3) , Hélène Magalon (2, 3) , Chloé A.-F. Annie-France Bourmaud (2, 3) , J. Henrich Bruggemann (2, 3)

1 IMBE - Institut méditerranéen de biodiversité et d'écologie marine et continentale
2 ENTROPIE [Réunion] - Ecologie marine tropicale dans les Océans Pacifique et Indien
3 LabEX CORAIL - Laboratoire d'Excellence CORAIL

A comprehensive inventory of global biodiversity would be greatly improved by automating methods for species delimitation. The Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule coalescent model have been proposed as means of increasing the rate of biodiversity description using single locus data. We applied these methods to explore the diversity within the Aglaopheniidae, a hydrozoan family with many species widely distributed across tropical and tem perate oceans. Our analyses revealed widespread cryptic diversity in this family, almost half of the morpho species presenting several independent evolutionary lineages, as well as support for cases of synonymy. For two common species of this family, Lytocarpia brevirostris and Macrorhynchia phoenicea, we compared the outputs to clustering analyses based on microsatellite data and to nuclear gene phylo genies. For L. brevirostris, microsatellite data were congruent with results of the species delimitation methods, revealing the existence of two cryptic species with Indo Pacific distribution. For M. phoenicea, all analyses confirmed the presence of two cryptic species within the South Western Indian Ocean. Our study suggests that the diversity of Aglaopheniidae might be much higher than assumed, likely related to low dispersal capacities. Sequence based species delimitation methods seem highly valuable to reveal cryptic diversity in hydrozoans; their application in an integrative framework will be very useful in describing the phyletic diversity of these organisms.

Mots clés: Automatic Barcode Gap Discovery; Generalized mixed Yule-coalescent model; Poisson tree processes; 16S; Microsatellites; Cnidarians

Domaines: Systématique, phylogénie et taxonomie

Bautisse Postaire, Hélène Magalon, Chloé A.-F. Annie-France Bourmaud, J. Henrich Bruggemann. Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa). Molecular Phylogenetics and Evolution, 2016, 105, pp.36-49. ⟨10.1016/j.ympev.2016.08.013⟩. ⟨hal-01359407⟩