Isolation and characterisation of 16 microsatellite loci from a widespread tropical hydrozoan, Lytocarpia brevirostris (Busk, 1852)

Bautisse Postaire (1, 2) , D. Aurelle (3) , Chloé A.-F. Annie-France Bourmaud (2, 1) , J. Henrich Bruggemann (2, 1) , Hélène Magalon (2, 1)

1 ECOMAR - Laboratoire d'Ecologie Marine
2 LabEX CORAIL - Laboratoire d'Excellence CORAIL
3 IMBE - Institut méditerranéen de biodiversité et d'écologie marine et continentale

We isolated and characterized 16 polymorphic microsatellite loci for Lytocarpia brevirostris (Aglaophe- niidae), a hydrozoan common in the tropical Indo-Pacific region. Four to 34 alleles per locus were detected at the Indo-Pacific scale. At the population level, observed (Ho) and expected (He) heterozygosities ranged across 0.100–0.625 and 0.097–0.597, respectively. Three markers showed significant deviation from Hardy–Weinberg equi- librium, all of them presenting null alleles. Linkage dis- equilibrium was detected in three pairs of loci among 120. These primers provide powerful tools for studying popu- lation genetic diversity and the implication of life cycle strategies on population differentiation in tropical hydro- zoans. This will be valuable for the conservation of coral reefs biodiversity and the design of marine protected areas.

Mots clés: Hydrozoa; Linkage Disequilibrium; Marine Protected Area; Microsatellite Locus; Reproductive Mode; Genetic Diversity; Coral Reef Biodiversity; Population Structure; Suspension Feeder; Population Differentiation; Allele Size; Null Allele; Diversity Index; Microsatellite Marker; Cryptic Specie; Titanium; Aglaopheniidae; Microsatellite; Lytocarpia brevirostris

Domaines: Systématique, phylogénie et taxonomie

Bautisse Postaire, D. Aurelle, Chloé A.-F. Annie-France Bourmaud, J. Henrich Bruggemann, Hélène Magalon. Isolation and characterisation of 16 microsatellite loci from a widespread tropical hydrozoan, Lytocarpia brevirostris (Busk, 1852). Conservation Genetics Resources, 2015, 7 (2), pp.505-507. ⟨10.1007/s12686-014-0407-1⟩. ⟨hal-01185722⟩